Rh antigen stability of mPEG modified red blood cells / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1020-1023, 2006.
Article
em Zh
| WPRIM
| ID: wpr-282740
Biblioteca responsável:
WPRO
ABSTRACT
The objective of study was to investigate the Rh antigen stability of mPEG-modified RBC. RBC membrane protein SDS-PAGE technology was used to analyze the combination of the mPEG modified RBC membrane protein with mPEG molecules; the RBC ghost coagulation test and 4 degrees C CPD-preserved modified RBC mixed with matched blood were used to observe the stability of RBC Rh antigen camouflaged by mPEG. The results showed that the blood groups of stored mPEG-modified RBC were kept consistency before or after simulating transfusion, i.e. mixture of modified RBC with matched bloods, while the plasma hemoglobin after simulating transfusion was not only within the normal range during the storage, but also less than that before simulating transfusion even after incubation at 37 degrees C. The electrophoresis pattern stained with iodine and Coomassie blue displayed the bands of mPEG combined with RBC membrane protein and the slow mobility of membrane protein. The hemagglutination of PEGylation RBC ghosts did not take place and mPEG still covered the antigen. In conclusion, mPEG-SPA can bind the erythrocyte with its extracted membrane protein in both ghosts and living erythrocytes.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Farmacologia
/
Polietilenoglicóis
/
Sistema do Grupo Sanguíneo Rh-Hr
/
Sangue
/
Alergia e Imunologia
/
Membrana Eritrocítica
/
Eritrócitos
/
Reação Transfusional
/
Isoanticorpos
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Ano de publicação:
2006
Tipo de documento:
Article