Febrile seizure, but not hyperthermia alone, induces the expression of heme oxygenase-1 in rat cortex / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 191-196, 2006.
Artigo
em Inglês
| WPRIM
| ID: wpr-282783
ABSTRACT
<p><b>BACKGROUND</b>Febrile seizure (FS) is the most common seizure disorders. Approximately one third of children with a febrile seizure have recurrent events. The mechanism of FS remains unclear. Heme oxygenase-1 (HO-1) is a member of the heat shock proteins family and can be induced in the brain by various stresses, including hyperthemia and seizure. This study aimed at investigating the changes of HO-1 in the cortex of rats after recurrent FS.</p><p><b>METHODS</b>FS in rats was induced ten times, once every 2 days. In a bath of warm water, developing rats were randomly divided into two groups control group (n = 16) and warm water-treated group (n = 50). The latter group was subdivided into hyperthermia group (n = 19) and FS group (n = 23). The expression and content of HO-1 mRNA in cortex were observed using in situ hybridization and quantitative reverse transcription-polymerase chain reaction (RT-PCR). The content of HO-1 protein in cortex was measured using Western blotting.</p><p><b>RESULTS</b>HO-1 mRNA expression of cortex neurons in FS group was markedly increased in comparison with those in hyperthermia and control groups (P = 0.00), however, there was no statistic difference between hyperthermia group and control group (P = 0.16). The relative amount of HO-1 mRNA in cortex in FS group was increased by 53.13% and 96% in comparison with those in hyperthermia group and control group respectively (P = 0.00), but there was no obvious difference between the later two groups (P = 0.051). Western blotting analysis showed that the HO-1 protein content in cortex in FS group was increased by 198% and 246% in comparison with those in hyperthermia group and control group respectively (P = 0.00). There was no obvious difference in HO-1 protein content between the later two groups (P = 0.09).</p><p><b>CONCLUSIONS</b>Recurrent FS in rats can cause the increase of HO-1 mRNA and protein in cortex which may be involved in the mechanism of FS. The short-time recurrent hyperthermia can not induce the increase of HO-1 mRNA and protein.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Recidiva
/
RNA Mensageiro
/
Córtex Cerebral
/
Ratos Sprague-Dawley
/
Convulsões Febris
/
Heme Oxigenase-1
/
Febre
/
Genética
Limite:
Animais
Idioma:
Inglês
Revista:
Chinese Medical Journal
Ano de publicação:
2006
Tipo de documento:
Artigo
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