GATA-3 expression in the development of anterior chamber associated immune deviation / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 2000-2004, 2005.
Artigo
em Inglês
| WPRIM
| ID: wpr-282832
ABSTRACT
<p><b>BACKGROUND</b>Anterior chamber associated immune deviation (ACAID) is characterized by a Th2 cell response. GATA-3 has been shown to be necessary for the activation of Th2 cells. This study was designed to examine the expression of GATA-3 in the development of ACAID.</p><p><b>METHODS</b>ACAID was induced by injection of 50 microg interphotoreceptor retinoid binding protein (IRBP) into the anterior chamber (AC) of Wistar rats. Delayed-type hypersensitivity (DTH) was evaluated on day 3, 7, 14, 21, 28 after IRBP inoculation. GATA-3 expression was detected using immunohistochemical staining. The expression of GATA-3 mRNA at different time points after AC injection of IRBP was assayed by reverse transcriptase polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>A significant DTH reaction was observed in Wistar rats on day 3 and 5 after IRBP inoculation. The DTH reaction was decreased 7 days after IRBP inoculation. GATA-3 expression was weak at both mRNA and protein levels in the normal spleen, but was significantly increased on day 5, 7, 14, and 21 after AC injection of IRBP.</p><p><b>CONCLUSION</b>The expression of GATA-3 is increased during ACAID, suggesting that GATA-3 may be involved in the development of ACAID.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Baço
/
Proteínas de Ligação ao Retinol
/
RNA Mensageiro
/
Ratos Wistar
/
Células Th2
/
Alergia e Imunologia
/
Fator de Transcrição GATA3
/
Proteínas do Olho
/
Genética
Limite:
Animais
Idioma:
Inglês
Revista:
Chinese Medical Journal
Ano de publicação:
2005
Tipo de documento:
Artigo
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