Long-term observation of large weight-bearing bone defect in goats repaired with tissue engineering technique / 南方医科大学学报
Journal of Southern Medical University
;
(12): 770-773, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-282920
ABSTRACT
<p><b>OBJECTIVE</b>To observe the long-term effect of tissue engineering-based repair of large weight-bearing bone defect in goats, and the final outcome of the scaffold material coral hydroxyapatite (CHAP) in vivo.</p><p><b>METHODS</b>Fifteen Chinese goats were subjected to operations to induce a 2-cm left tibial diaphyseal defect, which was filled subsequently with CHAP and bone marrow stromal stem cells (BMSCs). The repaired defects were evaluated by ECT, X-ray and histology in the early stage and at 6, 12, 18, and 24 months postoperatively.</p><p><b>RESULTS</b>ECT showed good bone regeneration and revascularization within 2 months postoperatively. X-ray and histology displayed eccentric and gradual bone regeneration in the early stage, and the tissue-engineered bone graft was firmly healed with the goat tibia. X-ray and histological examination at 6, 12, 18, 24 months postoperatively revealed moulding of the new bones and medullary cavity recanalization, and the structure of CHAP disappeared and gradually integrated into the new bones.</p><p><b>CONCLUSION</b>Tissue-engineered bone is capable of total repair of large bone defect in goats by forming normal functional new bones. CHAP can be eventually degraded completely and become the component of the newly generated bones.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Próteses e Implantes
/
Cirurgia Geral
/
Fraturas da Tíbia
/
Regeneração Óssea
/
Cabras
/
Células Cultivadas
/
Transplante de Medula Óssea
/
Substitutos Ósseos
/
Engenharia Tecidual
/
Métodos
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2006
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS