Your browser doesn't support javascript.
loading
Cloning, expression and immunocharacterization of the capsid protein of human Norwalk virus Guangzhou strain NVgz01 / 南方医科大学学报
Journal of Southern Medical University ; (12): 1410-1413, 2007.
Artigo em Chinês | WPRIM | ID: wpr-283118
ABSTRACT
<p><b>OBJECTIVE</b>To clone, express and characterize the capsid protein of human Norwalk virus Guangzhou strain NVgz01.</p><p><b>METHODS</b>On the basis of successful construction of full-genome clones and sequence analysis of human norovirus Guangzhou strain NVgz01, the full capsid gene was ligated into pET28a (+) for expression. After IPTG induction, the recombinant protein was purified through metal (Ni(2+)) chelating affinity chromatography. Western blotting and enzyme-linked immunosorbent assay (ELISA) were used to determine the antigenicity of the recombinant protein.</p><p><b>RESULTS</b>The recombinant capsid gene was overexpressed in E.coli, yielding the recombinant protein with relative molecular mass of 62x10(3) that was highly purified through metal (Ni(2+)) chelating affinity chromatography. IDEIA Norovirus Kit and immunoassay showed that the recombinant protein had good antigenicity.</p><p><b>CONCLUSION</b>The capsid gene of norovirus Guangzhou strain has been cloned and expressed, which can be useful for developing diagnostic reagents or vaccine of norovirus.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Plasmídeos / Ensaio de Imunoadsorção Enzimática / Expressão Gênica / Western Blotting / Clonagem Molecular / Vírus Norwalk / Proteínas do Capsídeo / Alergia e Imunologia / Genética Limite: Humanos Idioma: Chinês Revista: Journal of Southern Medical University Ano de publicação: 2007 Tipo de documento: Artigo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Plasmídeos / Ensaio de Imunoadsorção Enzimática / Expressão Gênica / Western Blotting / Clonagem Molecular / Vírus Norwalk / Proteínas do Capsídeo / Alergia e Imunologia / Genética Limite: Humanos Idioma: Chinês Revista: Journal of Southern Medical University Ano de publicação: 2007 Tipo de documento: Artigo