Study on pharmacokinetics of puerarin in rats following different methods of administration of Tongqiao Sanyu prescription / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study pharmacokinetic of puerarin in rats following different methods of administration of Tongqiao Sanyu prescription.</p><p><b>METHOD</b>Tongqiao Sanyu prescription was administered to rats by caudal vein injection, nasal administration and oral administration. Plasma samples were extracted with methanol and the plasma concentration of puerarin was analyzed by RP-HPLC. The pharmacokinetic parameters and bioavailability were calculated with Kinetica software.</p><p><b>RESULT</b>The main pharmacokinetic parameters were as follows AUC(0-infinity) of caudal vein injection was (787.99 +/- 70.44) mg x min x L(-1); AUC(0-infinity) of nasal administration was (376.56 +/- 93.93) mg x min x L(-1); AUC(0-infinity) and oral administration (The dose was decuple higher than that of caudal vein injection and nasal administration) was (491.18 +/- 110.64) mg x min x L(-1). The absolute bioavailability of puerarin was 47.78% by nasal administration and 6.23% by oral administration.</p><p><b>CONCLUSION</b>The bioavailability of nasal administration is higher than oral administration significantly, this result can provide some scientific foundantion for the method of administration and the reform of dosage form of Tongqiao Sanyu prescription.</p>