Activity evaluation of components and preparation of effective components group of xiaoxuming decoction for anti-cerebral ischemic / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>On basis of preliminary studies, to prepare the effective components group of Xiaoxuming decoction for anti-ischemic by combining chemical analysis with pharmacological activity screening.</p><p><b>METHOD</b>Free radical scavenging was assayed by DPPH method; the cell viability injured by hydrogen peroxide, L-glutamate, and hypoxia was determined by MTT assay; TBA method was used to determine mitochondrial lipid peroxidation.</p><p><b>RESULT</b>Comprehensive analysis of multi-target results, the comprehensive activities of 40% ethanol elution and the middle layer were the highest.</p><p><b>CONCLUSION</b>40% ethanol elution and the middle layer were proportionally mixed as the effective components group of Xiaoxuming decoction.</p>