Determination of underivatized glycine and proline in vinegar turtle shell by HPLC-ELSD / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2107-2109, 2011.
Artigo
em Chinês
| WPRIM
| ID: wpr-283247
ABSTRACT
<p><b>OBJECTIVE</b>To establish a method to determine the underivatized glycine (Gly) and proline (Pro) in vinegar turtle shell.</p><p><b>METHOD</b>An HPLC-ELSD method was conducted on a Prevail C18 column (4.6 mm x 250 mm, 5 microm) with the mobile phase of acetonitrile and 0.7% trifluoroacetic acid solution (containing 5.0 mmol x L(-1) heptafluorobutyric acid), and elution time was 15 min.</p><p><b>RESULT</b>The calibration curves were showed good linearity within the concentration range of 0.14-0.6 g x L(-1). The average recoveries were 101.2% and 102.5%, and RSD were 1.9% and 2.5%, respectively.</p><p><b>CONCLUSION</b>Since this method needs neither the special amino acid analyzer nor derivation of amino acid., it is efficient, simple and accurate., which could be used for quality control of vinegar turtle shell.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Controle de Qualidade
/
Padrões de Referência
/
Tartarugas
/
Prolina
/
Química
/
Reprodutibilidade dos Testes
/
Cromatografia Líquida de Alta Pressão
/
Exoesqueleto
/
Glicina
Limite:
Animais
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2011
Tipo de documento:
Artigo
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