PCR, clone and sequence analysis of rDNA-ITS of Nelumbo nucifera from different geographical origins in China / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 671-675, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-283412
ABSTRACT
<p><b>OBJECTIVE</b>To provide DNA molecular marker for identification of Nelumbo nucifera by exploring the differences of nrDNA-ITS sequence of N. nucifera originated from different habitats.</p><p><b>METHOD</b>To compare nrDNA-ITS base sequence using specific PCR-ITS.</p><p><b>RESULT</b>The completed sequence of ITS and 5.8 S rDNA, and the partial sequences of 18S rDNA and 26S rDNA, totally 750 bp, from N. nucifera were obtained. The differences among N. nucifera from different habitats and from different cultivars were found.</p><p><b>CONCLUSION</b>The method can be used to identify N. nucifera among different species and to distinguish their fakes. It provided the basis for identifying N. nucifera from different geographical regions by comparison of their ITS sequences.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Filogenia
/
Plantas Medicinais
/
Especificidade da Espécie
/
RNA Ribossômico
/
RNA Ribossômico 18S
/
Sequência de Bases
/
Desoxirribonuclease EcoRI
/
Desoxirribonucleases de Sítio Específico do Tipo II
/
Química
/
China
Tipo de estudo:
Estudo prognóstico
País/Região como assunto:
Ásia
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2007
Tipo de documento:
Artigo
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