In vitro studies on asexual embryos and regenerated plantlets obtained from leaf organ of Panax notoginseng / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study and improve the tissue culture technology of Panax notoginseng.</p><p><b>METHOD</b>Using the callus of leaf blade and leafstalk of P. notogingseng as explants, MS + 2, 4-D 1.5 mg x L(-1) as basal medium, the formation of asexual embryos was induced by added LFS, BA, KT or ZT 0.5 mg x L(-1), and cultured in dark. It cultured then in 2000 lx of illumination for 10-12 h x d(-1) to induce the asexual embryos germinating and developing to be the regenerated-plantlet.</p><p><b>RESULT AND CONCLUSION</b>Only the medium added with LFS could induce the formation of asexual embryos, and made it developed to be regenerated-plantlet. The inducing ratio of asexual embryos reached about 85%, and 30% of asexual embryos could grow and develop as robust regenerated-plantlets.</p>