The role of Egr-1 and NF-kappaB in the pathogenesis of silicosis: an in-vitro study / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 363-367, 2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-283506
ABSTRACT
<p><b>OBJECTIVE</b>To study the correlation between the expression of Egr-1 and NF-kappaB and the up-regulation of TNF-alpha and TGF-beta1 in macrophages after stimulation by silica in-vitro.</p><p><b>METHODS</b>Macrophages were treated with antibodies against Egr-1 and NF-kappaB and antisense oligonucleotides. The level of TNF-alpha protein in the cell supernatant was then measured using enzyme-linked immunoadsorbent assay (ELISA). The expression of TGF-beta1 protein was detected by immunocytochemistry. The expression of TNF-alpha and TGF-beta1 mRNAs was also monitored by reverse transcriptase-polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>Compared with silica-stimulated macrophages untreated with antibodies, the cells treated with 10 micro g/ml of Egr-1 or NF-kappaB antibodies were associated with reduced expression of TNF-alpha and TGF-beta1 proteins and mRNAs (P < 0.05). Compared with silica-stimulated untransfected group, the antisense group was associated with obvious reduction in the expression of TNF-alpha and TGF-beta1 proteins and mRNAs (P < 0.05).</p><p><b>CONCLUSION</b>The expression of TNF-alpha and TGF-beta1 mRNAs and proteins are associated with activation of Egr-1 and NF-kappaB in macrophages, after stimulation by silica. It is possible that the corresponding antibodies and antisense oligonucleotides may become a potential therapeutic tool in the management of silicosis in the future.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Silicose
/
Fatores de Transcrição
/
RNA Mensageiro
/
Células Cultivadas
/
NF-kappa B
/
Oligonucleotídeos Antissenso
/
Fator de Crescimento Transformador beta
/
Fator de Necrose Tumoral alfa
/
Proteínas Imediatamente Precoces
Tipo de estudo:
Estudo de etiologia
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Pathology
Ano de publicação:
2004
Tipo de documento:
Artigo
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