beta-elemene enhances aclarubicin-induced apoptotic effect in HL-60 cells and its mechanism / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 821-824, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-283899
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effects of beta-elemene combined with aclarubicin on the induction of HL-60 cell apoptosis and its mechanisms in antileukemia therapy.</p><p><b>METHODS</b>HL-60 cells were treated for 20 hours with different dose of aclarubicin (0.05, 0.10, 0.25 microg/ml) or with different concentrations of beta-elemene (10, 20, 40 microg/ml) in the presence or absence of aclarubicin (0.10 microg/m). The apoptotic rate was analyzed by flow cytometry (FCM), the productions of PGE2 in culture supernatants was detected by competitive ELISA and the expressions of COX-2 and NF-kappaB activity in HL-60 cells by Western blot.</p><p><b>RESULTS</b>Lower concentration of aclarubicin (0.05, 0.10 microg/ml) didn't affect apoptotic rate, and COX-2, NF-kappa B and PGE2 expression on HL-60 cells. Combined treatment of beta-elemene and aclarubicin (0.10 microg/ml) enhanced the apoptotic effect and down-regulated COX-2, NF-kappaB and PGE2 expressions. There was a positive correlation between the effects and beta-elemene concentrations.</p><p><b>CONCLUSION</b>beta-elemene enhances aclarubicin-mediated apoptotic effect, down-regulation of COX-2 and their inducing products PGE2 in HL-60 cells by suppressing activitation of NF-kappaB.</p>
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Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Aclarubicina
/
Regulação para Baixo
/
NF-kappa B
/
Apoptose
/
Células HL-60
/
Linhagem Celular Tumoral
/
Metabolismo
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Hematology
Ano de publicação:
2009
Tipo de documento:
Artigo
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