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Study on Wnt and Notch signalling involves in regulation of hematopoietic microenvironment / 中华血液学杂志
Chinese Journal of Hematology ; (12): 799-803, 2009.
Artigo em Chinês | WPRIM | ID: wpr-283904
ABSTRACT
<p><b>OBJECTIVE</b>To explore the mechanism of Wnt and Notch pathway involved modulating time and spatial restricted hematopoiesis.</p><p><b>METHODS</b>Murine hematopoietic stem and progenitor cells (HSPCs) were isolated from bone marrow (BM) by using c-kit microbeads. E10.5 aorta-gonad-mesonephros (AGM), E12.5, E14.5, E16.5 fetal liver (FL) and adult BM derived stromal cells (StroCs) were isolated and co-cultured with c-kit(+)HSPCs. The floating cells in co-culture system were sorted and counted by FACS. Gene expressions of Wnt and Notch pathway were detected by quantitative PCR and protein expressions by immunostaining.</p><p><b>RESULTS</b>Co-culturing HSPCs with AGM and FL-derived StroCs resulted in an expansion of c-kit(+)population from 0.4 x 10(5)/well to (19.2 +/- 3.2) x 10(5)/well and (26.8 +/- 5.4) x 10(5)/well, respectively, being greater than that with BM-derived StroCs (P < 0.05). The percentage of c-kit(+)cells detected in AGM- and BM- derived StroCs culture system was (75.2 +/- 7.1)%, (74.1 +/- 6.2)% respectively, being higher than FL- derived StroCs culture system (63.4 +/- 5.3)% (P < 0.05). Wnt and Notch pathway genes expression varied at different phases of hematopoiesis. Wnt was highly expressed in AGM and FL derived StroCs, and, Notch did in AGM and BM derived StroCs.</p><p><b>CONCLUSION</b>Wnt and Notch pathway are important modulators in regulating time and spatial restricted hematopoiesis.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Aorta / Células-Tronco Hematopoéticas / Células Estromais / Técnicas de Cocultura / Biologia Celular / Hematopoese / Mesonefro Limite: Animais / Humanos Idioma: Chinês Revista: Chinese Journal of Hematology Ano de publicação: 2009 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Aorta / Células-Tronco Hematopoéticas / Células Estromais / Técnicas de Cocultura / Biologia Celular / Hematopoese / Mesonefro Limite: Animais / Humanos Idioma: Chinês Revista: Chinese Journal of Hematology Ano de publicação: 2009 Tipo de documento: Artigo