Studies on the integration of hepatitis B virus DNA sequence in human sperm chromosomes / 亚洲男科学杂志(英文版)
Asian Journal of Andrology
;
(6): 209-212, 2002.
Artigo
em Inglês
| WPRIM
| ID: wpr-284047
ABSTRACT
<p><b>AIM</b>To study the integration of hepatitis B virus (HBV) DNA into sperm chromosomes in hepatitis B patients and the features of its integration.</p><p><b>METHODS</b>Sperm chromosomes of 14 subjects (5 healthy controls and 9 HB patients, including 1 acute hepatitis B, 2 chronic active hepatitis B, 4 chronic persistent hepatitis B, 2 HBsAg chronic carriers with no clinical symptoms) were prepared using interspecific in vitro fertilization between zona-free hamster oocytes and human spermatozoa. Fluorescence in situ hybridization (FISH) to sperm chromosome spreads was carried out with biotin-labeled full length HBV DNA probe to detect the specific HBV DNA sequences in the sperm chromosomes.</p><p><b>RESULTS</b>Specific fluorescent signal spots for HBV DNA were seen in sperm chromosomes of one patient with chronic persistent hepatitis B. In 9 (9/42) sperm chromosome complements containing fluorescent signal spots, one presented 5 obvious FISH spots and the others 2 to 4 signals. The fluorescence intensity showed significant difference among the signal spots. The distribution of signal sites among chromosomes seems to be random.</p><p><b>CONCLUSION</b>HBV could integrate into human sperm chromosomes. Results suggest that the possibility of vertical transmission of HBV via the germ line to the next generation is present.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Espermatozoides
/
Virologia
/
Vírus da Hepatite B
/
Cromossomos Humanos
/
Integração Viral
/
Hibridização in Situ Fluorescente
/
Transmissão Vertical de Doenças Infecciosas
/
Genética
/
Hepatite B
Limite:
Adulto
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Asian Journal of Andrology
Ano de publicação:
2002
Tipo de documento:
Artigo
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