Antitumor effect of baicalin on rat brain glioma / 中华肿瘤杂志
Chinese Journal of Oncology
; (12): 11-16, 2013.
Article
em Zh
| WPRIM
| ID: wpr-284248
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the therapeutic mechanism of baicalin on rat brain glioma.</p><p><b>METHODS</b>Deep brain glioma models were established by injection of glioma cell line C6 cells into the brain of Wistar rats. The rats at 7 days after modeling were randomly divided into tumor control group (0.9% NaCl solution 30 mg×kg(-1)×d(-1) gavage)and experimental groups. The experimental rats was divided into 3 groups: low dose group (50 mg×kg(-1)×d(-1)), middle dose group (100 mg×kg(-1)×d(-1)) and high dose group (200 mg×kg(-1)×d(-1)), given the baicalin by gavage. Pathological and electron microscopic changes were observed. The expressions of p53 and Bcl-2 were determined by immunohistochemistry, and the changes of MRI, the average survival time and body weight of the rats in each group after treatments were analyzed.</p><p><b>RESULTS</b>Compared with the control group, the tumor diameter and volume of high dose group rats before sacrifice were significantly reduced (P < 0.01), and the survival time was significantly prolonged (P < 0.01). Immunohistochemistry revealed strong positive expression rate of mutant p53 (84.47 ± 3.74)% and moderately positive rate (47.28 ± 2.38)% in the control group, significantly higher than that in the negative group (12.91 ± 1.07)% (P < 0.01). The positive rate of mutant p53 of the high dose group was (46.42 ± 2.19)%, significantly lower than that of the control group (84.47 ± 3.74)% (P < 0.01). The expression rate of Bcl-2 in the control group was strongly positive (86.51 ± 4.17)% and moderate positive (48.19 ± 2.11)%, significantly higher than that of the negative group (10.36 ± 1.43)% (P < 0.01). Electron microscopy revealed that baicalin caused damages of the cell nuclei and organelles in the gliomas.</p><p><b>CONCLUSIONS</b>Baicalin has significant inhibitory effect on glioma in vivo, and its mechanism may be related to cell apoptosis induced by down-regulated expression of mutant p53, but not related with Bcl-2 expression.</p>
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Índice:
WPRIM
Assunto principal:
Patologia
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Farmacologia
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Flavonoides
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Neoplasias Encefálicas
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Imageamento por Ressonância Magnética
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Distribuição Aleatória
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Regulação para Baixo
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Núcleo Celular
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Proteína Supressora de Tumor p53
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Ratos Wistar
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Oncology
Ano de publicação:
2013
Tipo de documento:
Article