Establishment of a high-throughput respiratory virus detection technology without RNA purification and reverse transcription / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
; (6): 24-28, 2013.
Article
em Zh
| WPRIM
| ID: wpr-284310
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a convenient and high-throughput respiratory virus detection method to facilitate epidemiological viral monitoring.</p><p><b>METHODS</b>We used high-throughput microsphere-based flexible multi-analyte profiling technology (xMAP) coupled with signal amplification molecules to simultaneously detect RNAs of 8 viruses including influenza viruses A and B, parainfluenza viruses type 1, 2 and 3, respiratory syncytial viruses A and B, and metapneumovirus in a 96-well plate format. The sensitivity and specificity of the method for the synthetic viral RNAs were evaluated.</p><p><b>RESULTS</b>There was no cross-reactivity among the 8 respiratory viral target RNAs. The detection limits for the 8 viral in intro-transcribed RNAs ranged from 1204 to 4695 RNA copies.</p><p><b>CONCLUSION</b>We establish a sensitive, specific, convenient, and high-throughput multiplex detection method suitable for detecting multiple respiratory viral RNAs for epidemiological viral monitoring.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Vírus da Influenza A
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Vírus da Influenza B
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Vírus Sinciciais Respiratórios
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Sistema Respiratório
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Virologia
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RNA Viral
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Sensibilidade e Especificidade
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Respirovirus
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Técnicas de Amplificação de Ácido Nucleico
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Metapneumovirus
Tipo de estudo:
Diagnostic_studies
Idioma:
Zh
Revista:
Acta Academiae Medicinae Sinicae
Ano de publicação:
2013
Tipo de documento:
Article