Establishment of a high-throughput respiratory virus detection technology without RNA purification and reverse transcription / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 24-28, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-284310
ABSTRACT
<p><b>OBJECTIVE</b>To establish a convenient and high-throughput respiratory virus detection method to facilitate epidemiological viral monitoring.</p><p><b>METHODS</b>We used high-throughput microsphere-based flexible multi-analyte profiling technology (xMAP) coupled with signal amplification molecules to simultaneously detect RNAs of 8 viruses including influenza viruses A and B, parainfluenza viruses type 1, 2 and 3, respiratory syncytial viruses A and B, and metapneumovirus in a 96-well plate format. The sensitivity and specificity of the method for the synthetic viral RNAs were evaluated.</p><p><b>RESULTS</b>There was no cross-reactivity among the 8 respiratory viral target RNAs. The detection limits for the 8 viral in intro-transcribed RNAs ranged from 1204 to 4695 RNA copies.</p><p><b>CONCLUSION</b>We establish a sensitive, specific, convenient, and high-throughput multiplex detection method suitable for detecting multiple respiratory viral RNAs for epidemiological viral monitoring.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Vírus da Influenza A
/
Vírus da Influenza B
/
Vírus Sinciciais Respiratórios
/
Sistema Respiratório
/
Virologia
/
RNA Viral
/
Sensibilidade e Especificidade
/
Respirovirus
/
Técnicas de Amplificação de Ácido Nucleico
/
Metapneumovirus
Tipo de estudo:
Estudo diagnóstico
Idioma:
Chinês
Revista:
Acta Academiae Medicinae Sinicae
Ano de publicação:
2013
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS