Determination of vetatramine in Veratrum nigrum by HPLC-ELSD / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To develop an HPLC-ELSD method for determination of vetatramine. in Veratrum nigrum.</p><p><b>METHOD</b>The analy fical column was Shim-pack ODS - C18 (4.6 mm x 250 mm, 4 microm) column, the mobile phase was acetonitrile-water (containing 0.1% triethylamine) (5050), at a flow rate of 0.8 mL x min(-1). The temperature of drift tube was 90 degrees C and the gas flow was at the rate of 2.5 L x min(-1).</p><p><b>RESULT</b>The calibration curve was linear in the range of 0.36-3.6 microg (r = 0.999 8). The average recovery was 100.9% (RSD 2.3%, n = 6). The contents of veratramine in Veratrum nigrum. from the ten different sources were determined.</p><p><b>CONCLUSION</b>The method may be used as a accurate and reproducible way to determine the content of veratramine in V. nigrum.</p>