Purification of total flavonoid glycosides from Hypericum perforatum by macroporous adsorption resin with on-line detection system / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 769-821, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-284398
ABSTRACT
<p><b>OBJECTIVE</b>To design a macroporous resin chromatography system with on-line detector which can be used to purify total flavonoid glycosides from Hypericum perforatum.</p><p><b>METHOD</b>Compared with the present detection methods, the ultraviolet detector which can be applied to flow liquid, and a self-made shunt were applied to design the on-line detection system. This system was successfully applied to purify the total flavonoid glycosides from H. perforatum.</p><p><b>RESULT</b>This on-line detection system was simple and feasible, and the results were accurate and reliable. The purity of total flavonoid glycosides from H. perforatum. separated by this system was higher than 90%.</p><p><b>CONCLUSION</b>This technique provides a simple and reliable on-line detection method for industrial chromatography purification of TCM extracts, which is the basis of automatic manufactory of TCM purification.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Controle de Qualidade
/
Resinas Vegetais
/
Raios Ultravioleta
/
Flavonoides
/
Sistemas On-Line
/
Química
/
Reprodutibilidade dos Testes
/
Cromatografia
/
Porosidade
/
Sensibilidade e Especificidade
Tipo de estudo:
Estudo diagnóstico
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2008
Tipo de documento:
Artigo
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