Activation of protein kinase A alters subnuclear distribution pattern of human steroidogenic factor 1 in living cells / 中华医学杂志(英文版)
Chinese Medical Journal
; (24): 1017-1022, 2004.
Article
em En
| WPRIM
| ID: wpr-284858
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>BACKGROUND</b>The aim of this study was to identify the subnuclear distribution pattern of human orphan nuclear receptor steroidogenic factor 1 (SF-1) in living cells with and without the activation of protein kinase A (PKA) signal pathway, and thus try to explain the unknown mechanism by which PKA potentiates SF-1 transactivation.</p><p><b>METHODS</b>Full-length cDNAs of wild type and a naturally occurring mutant (G35E) human SF-1 were cloned and fused with green fluorescent protein (GFP). Subcellular distribution pattern of human SF-1 in living cells, whose PKA signaling was either activated or not, was studied by laser confocal microscopy after the validity of the gene sequence was confirmed.</p><p><b>RESULTS</b>The transactivation ability of the GFP-SF-1 chimeric protein was highly conserved. Wild type human SF-1 diffused homogeneously within the nuclei of cells when PKA was not active, and converged to clear foci when PKA was activated. Mutant SF-1 diffused within the nuclei even in the presence of PKA activation, surprisingly aggregating as fluorescent dots inside the nucleoli, a phenomenon not altered by PKA.</p><p><b>CONCLUSIONS</b>Activation of PKA causes wild type, but not mutant SF-1 to alter its subnuclear distribution pattern to a transactivationally active form (foci formation). This finding may throw new light on the mechanism by which PKA activates the orphan nuclear receptor.</p>
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Índice:
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Assunto principal:
Farmacologia
/
Fisiologia
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Fatores de Transcrição
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Colforsina
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Compartimento Celular
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Ativação Transcricional
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Núcleo Celular
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Células Cultivadas
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Química
/
Receptores Citoplasmáticos e Nucleares
Tipo de estudo:
Prognostic_studies
Limite:
Female
/
Humans
Idioma:
En
Revista:
Chinese Medical Journal
Ano de publicação:
2004
Tipo de documento:
Article