Advancement in the methods for gene dosage analysis / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 76-79, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-285029
ABSTRACT
Gene dosage determination is increasingly important for the study of both genome variation and rearrangement associated with complex diseases. Large genomic duplications and deletions are increasingly found as the causes. Methods such as PCR or sequencing are usually qualitative rather than quantitative. Thus, these methods can not detect large genomic duplications or deletions. Therefore, searching for a gene dosage method which is reliable, sensitive and high-throughput becomes imperative. Many high-performance technologies have been developed for gene dosage analyses in the recent years. There are generally three categories of methods including cytogenetic, Southern or dot blotting, or PCR amplification. Recent development in these techniques have been introduced and discussed in this review, which will help people to choose a suitable method for different research.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Southern Blotting
/
Reação em Cadeia da Polimerase
/
Deleção de Genes
/
Dosagem de Genes
/
Duplicação Gênica
/
Análise Citogenética
/
Genética
/
Métodos
Tipo de estudo:
Pesquisa qualitativa
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Medical Genetics
Ano de publicação:
2007
Tipo de documento:
Artigo
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