Your browser doesn't support javascript.
loading
hPARP1 genetic polymorphism in southern Chinese Han and Miao populations / 中华医学遗传学杂志
Chinese Journal of Medical Genetics ; (6): 578-580, 2006.
Artigo em Chinês | WPRIM | ID: wpr-285071
ABSTRACT
<p><b>OBJECTIVE</b>To study hPARP1 genetic polymorphism in southern Chinese Han and Miao populations.</p><p><b>METHODS</b>Blood samples from 187 and 210 southern healthy Han and Miao populations were collected. The mutations of exons 12,13,16 and 17 of hPARP1 gene were investigated by PCR-single-strand conformation polymorphism(SSCP).</p><p><b>RESULTS</b>Fragments of 253 bp,313 bp,175 bp,362 bp within exons 12,13,16 and 17 respectively of hPARP1 gene were amplified by multiple PCR. An SSCP variant in exons 12,13,16 and 17 of PARP1 gene in 187 healthy Han and 210 healthy Miao individuals was identified. Seven single-base substitutions compared with the sequence of PARP1 gene were identified a T to C transition in exon 12 (Phe548Ser), a G to T transition in exon 13 (Ala683Ser), a G to T transition in exon 16 (Asp798Tyr), and a A to G transition in exon 17 (His808Arg).</p><p><b>CONCLUSION</b>There were polymorphism sites in exons 12,13,16,17 of hPARP1 gene in southern Chinese Han and Miao populations; these results may be useful for the establishment of PARP1 genotyping, and these newly described PARP1 alleles would be advantageous indicators for population studies.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: China / Reação em Cadeia da Polimerase / Éxons / Poli(ADP-Ribose) Polimerases / Polimorfismo Conformacional de Fita Simples / Polimorfismo de Nucleotídeo Único / Alelos / Poli(ADP-Ribose) Polimerase-1 / Frequência do Gene / Genética Limite: Adulto / Feminino / Humanos / Masculino País/Região como assunto: Ásia Idioma: Chinês Revista: Chinese Journal of Medical Genetics Ano de publicação: 2006 Tipo de documento: Artigo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: China / Reação em Cadeia da Polimerase / Éxons / Poli(ADP-Ribose) Polimerases / Polimorfismo Conformacional de Fita Simples / Polimorfismo de Nucleotídeo Único / Alelos / Poli(ADP-Ribose) Polimerase-1 / Frequência do Gene / Genética Limite: Adulto / Feminino / Humanos / Masculino País/Região como assunto: Ásia Idioma: Chinês Revista: Chinese Journal of Medical Genetics Ano de publicação: 2006 Tipo de documento: Artigo