Characteristics of IGF-II gene imprinting in twin placentas / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 497-501, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-285092
ABSTRACT
<p><b>OBJECTIVE</b>To compare insulin-like growth factor II (IGF-II) gene imprinting in twin placentas with singleton ones and to determine whether imprinting was influenced by assisted reproductive technology, zygosity and fetal sex.</p><p><b>METHODS</b>One hundred and sixty cases of twin placentas and 42 cases of singleton ones were recruited. Allele-specific IGF-II expression was determined by reverse transcription-PCR combined with analysis of an Apa I-sensitive restriction fragment length polymorphism.</p><p><b>RESULTS</b>Although the incidence of IGF-II imprinting loss was higher in normal twin placentas than in singleton ones (20.6% vs 8.7%), there was no statistical significance. There were no significant differences between twins conceived by assisted reproductive technology and those conceived spontaneously (17.9% vs 24.4%), and between dizygotic and monozygotic twins (22.4% vs 16.7%). The incidence of IGF-II imprinting loss in placenta of female twins was statistically higher than that of male ones (26.4% vs 9.8%).</p><p><b>CONCLUSION</b>The risk of IGF-II gene imprinting loss is higher in female twins and has no relationship with assisted reproductive technology and zygosity.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Placenta
/
Polimorfismo Genético
/
Gêmeos
/
Fator de Crescimento Insulin-Like II
/
Idade Materna
/
Impressão Genômica
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Genética
/
Metabolismo
Limite:
Adulto
/
Feminino
/
Humanos
/
Masculino
/
Recém-Nascido
/
Gravidez
Idioma:
Chinês
Revista:
Chinese Journal of Medical Genetics
Ano de publicação:
2006
Tipo de documento:
Artigo
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