Stereotactic injection of shrna GSK-3β-AAV promotes axonal regeneration after spinal cord injury / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences)
;
(6): 548-553, 2016.
Artigo
em Inglês
| WPRIM
| ID: wpr-285231
ABSTRACT
Evidence suggested that glycogen synthase kinase-3β (GSK-3β) is involved in Nogo-66 inhibiting axonal regeneration in vitro, but its effect in vivo was poorly understood. We showed that stereotactic injection of shRNA GSK-3β-adeno associated virus (GSK-3β-AAV) diminished syringomyelia and promoted axonal regeneration after spinal cord injury (SCI), using stereotactic injection of shRNA GSK-3β-AAV (tested with Western blotting and RT-PCR) into the sensorimotor cortex of rats with SCI and by the detection of biotin dextran amine (BDA)-labeled axonal regeneration. We also determined the right position to inject into the sensorimotor cortex. Our findings consolidate the hypothesis that downregulation of GSK-3β promotes axonal regeneration after SCI.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Traumatismos da Medula Espinal
/
Axônios
/
Siringomielia
/
Terapêutica
/
Dependovirus
/
RNA Interferente Pequeno
/
Córtex Sensório-Motor
/
Glicogênio Sintase Quinase 3 beta
/
Genética
Limite:
Animais
/
Humanos
Idioma:
Inglês
Revista:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Ano de publicação:
2016
Tipo de documento:
Artigo
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