Effects of ingredients from Chinese herbs with nature of cold or hot on expression of TRPV1 and TRPM8 / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1594-1598, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-285320
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of the ingredients from Chinese herbs with the nature of cold or hot on the expression of TRPV1 and TRPM8.</p><p><b>METHOD</b>The effects of ingredients from herbs on primary culture DRG neurons are observed in vitro. The expression quantity of gene is detected by the method of real time PCR. the 2 (-deltadeltaCT) method is applied to analyze the data.</p><p><b>RESULT</b>Ingredients from herbs with the nature of cold up-regulate the expression level of TRPV1 and down-regulate that of TRPM8, especially under the temperature condition of 39 degrees C; while ingredients from herbs with the nature of hot up-regulate the expression level of TRPM8 and down-regulated that of TRPV1, which is more significant under the temperature condition of 19 degrees C.</p><p><b>CONCLUSION</b>The regulatory changes of TRPV1 and TRPM8 mRNA expression induced by the chemical ingredients might be related to the cold and hot natures of the herbs from which the ingredients are extracted. And this could be one of the therapeutic mechanisms for the treatment of Chinese herbal medicines to cold- and heat-related diseases.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Medicamentos de Ervas Chinesas
/
Expressão Gênica
/
Ratos Sprague-Dawley
/
Canais de Cátion TRPM
/
Canais de Cátion TRPV
/
Genética
/
Metabolismo
Limite:
Animais
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2010
Tipo de documento:
Artigo
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