Retros Flt-1 decelerates the growth of a murine experimental osteosarcoma / 中华外科杂志
Chinese Journal of Surgery
;
(12): 746-751, 2011.
Artigo
em Chinês
| WPRIM
| ID: wpr-285650
ABSTRACT
<p><b>OBJECTIVE</b>To examine the influence of vascular endothelial growth factors (VEGF) in controlling the growth of an experimental osteosarcoma in mice by performing retrovirus-mediated sFlt-1 gene modification.</p><p><b>METHODS</b>From March to October 2010 human osteosarcoma G-292 cells were in vitro infected with retroviral vectors encoding soluble Flt-1 or LacZ gene before transplanted into proximal tibiae of immune deficient SCID mice to establish experimental orthotopic osteosarcoma. Daily observation and biweekly microCT were performed to monitor tumor development and progression till sacrifice at 8 weeks after tumor cell inoculation for histological and molecular analyses.</p><p><b>RESULTS</b>Successful transgene expression was confirmed in the culture media of sFlt-1 transduced G-292 cells using ELISA, and with positive X-gal staining of the LacZ transduced cells. Noteworthy tumors were grown in all mice on the tibiae receiving G-292 cell inoculation, with clear detection on microCT images starting 2 weeks after inoculation. Over the time period, tumors derived from sFlt-1 transduced G-292 cells were distinctively smaller in size compared to the ones from wide-type G-292 and G-292-LacZ cells. Histology showed typical osteosarcoma characteristics including severe cellular pleomorphism, bone erosions, and neo-vascularization. Real-time polymerase chain reaction indicated significantly higher sFlt-1 expression in sFlt-1 transduced groups than the wild-type G-292 or LacZ treated groups. Strong expression of oncogenes c-myc and c-fos were also obvious, along with the expression of VEGF in the primary tumor tissue.</p><p><b>CONCLUSION</b>Retrovirus-mediated sFLT-1 gene modification decelerates the osteosarcoma tumor growth in this murine model.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Retroviridae
/
Neoplasias Ósseas
/
Osteossarcoma
/
Camundongos SCID
/
Transgenes
/
Receptor 1 de Fatores de Crescimento do Endotélio Vascular
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Linhagem Celular Tumoral
/
Fator A de Crescimento do Endotélio Vascular
/
Vetores Genéticos
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
/
Feminino
/
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Surgery
Ano de publicação:
2011
Tipo de documento:
Artigo
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