Combined effects of NEL-like type 1 gene and zoledronate in preventing collapse of the femoral head / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 553-560, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-285961
ABSTRACT
<p><b>OBJECTIVE</b>To determine if combined therapy consisting of NEL-like type 1 gene (NELL-1) and zoledronate can prevent the collapse of the femoral head and stimulate the new bone formation in an animal model of osteonecrosis.</p><p><b>METHODS</b>Ischemic osteonecrosis was surgically induced in 24 SD rats, whicih were equally randomly divided into three groups combination group, treated with both NELL-1 and zoledronate; sham operation group; and placebo group, treated with normal saline solution. The animals were killed 5 weeks after surgery. Radiography, MicroCT, histology, and immunohistochemistry were performed to analyze the results.</p><p><b>RESULTS</b>Morphologically, the femoral head was at good shape in the combination group, while mildly flattened femoral head was seen in the placebo group. No heterotopic ossifications were observed in each group. MicroCT assessment showed significantly higher total and bone mineral volume in the combination group than in the placebo group (P<0.01), whereas no such significant difference was found when compared with the sham operation group(P>0.05). Histological assessment showed more active osteoblast activity and reduced osteoclast activity in the combination group compared with placebo group.</p><p><b>CONCLUSION</b>A combination of NELL-1 and zoledronate can decrease the femoral head deformity while stimulating bone formation in a traumatic rat osteonecrois model, showing a potential to reverse the osteonecrosis.</p>
Texto completo:
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Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Distribuição Aleatória
/
Ratos Sprague-Dawley
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Usos Terapêuticos
/
Difosfonatos
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Tratamento Farmacológico
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Necrose da Cabeça do Fêmur
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Imidazóis
/
Proteínas do Tecido Nervoso
Limite:
Animais
Idioma:
Chinês
Revista:
Acta Academiae Medicinae Sinicae
Ano de publicação:
2013
Tipo de documento:
Artigo
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