Co-occlusion of foreign protein into polyhedra with BmNPV polyhedrin / 病毒学报
Chinese Journal of Virology
;
(6): 366-371, 2011.
Artigo
em Chinês
| WPRIM
| ID: wpr-286027
ABSTRACT
In order to make clear the packing mechanism of the BmNPV polyhedra, a polyhedrin gene negative recombinant baculovirus, vBmBac(polh-)-5B-EGFP, expressing EGFP was constructed, and used to infect BmN cells jointly with wild-type BmNPV. Fluorescent microscopic observation demonstrated that EGFP and polyhedrin were expressed simultaneously, and the EGFP expression and polyhedra formation occurred in most of the jointly infected cells. Analysis of the purified polyhedra from jointly infected BmN cells showed that the foreign proteins were present in the polyhedra. The results indicated that BmNPV polyhedrin could incorporate proteins other than viral proteins into the polyhedra. It implies that a nonspecific recognition mechanism exists in the embedment of BmNPV polyhedra.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Bombyx
/
Expressão Gênica
/
Proteínas Estruturais Virais
/
Nucleopoliedrovírus
/
Montagem de Vírus
/
Proteínas de Fluorescência Verde
/
Genética
/
Metabolismo
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Virology
Ano de publicação:
2011
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS