Construction and rescue of rabies virus mutant strain SRV9 / 病毒学报
Chinese Journal of Virology
;
(6): 345-350, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-286114
ABSTRACT
To construct a rabies virus mutant, the psi region was replaced by the coding region of human cytochrome c gene, and the coding region for cytoplasmic domain of glycoprotein G was deleted in the full-length of genomic cDNA of rabies virus strain SRV9. The mutant plasmid and the plasmids with N, P, L and G structural proteins of wild type SRV9 were co-transfected into BHK-21 cells. It was shown by IFA that there were many specific fluorescence in the BHK-21 cells, and typical rabies virus virions were observed by electronic microscope. These results demonstrated that the mutant rabies virus was successfully rescued. The genetically modified SRV9 stain has promise to provide invaluable experimental tool to develop attenuated live rabies vaccine.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Vírus da Raiva
/
DNA Viral
/
Linhagem Celular
/
Microscopia Imunoeletrônica
/
Genoma Viral
/
DNA Complementar
/
Genética
/
Mutação
Limite:
Animais
/
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Virology
Ano de publicação:
2010
Tipo de documento:
Artigo
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