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Porcine follistatin cDNA cloning and expression in Escherichia coli / 生物工程学报
Chinese Journal of Biotechnology ; (12): 677-681, 2006.
Artigo em Chinês | WPRIM | ID: wpr-286228
ABSTRACT
The total RNA was extracted from porcine ovary. Porcine Follistatin cDNA was cloned by RT-PCR. Complete porcine follistatin cDNA coding sequences are presented including 1038 bp of open reading frame. The purified porcine follistatin cDNA was inserted into pGEX-4T-3 vector to construct the prokaryotic fusion protein expression vector. The recombinant expression plasmid was transformed into BL21 (DE3) and expression was induced by IPTG. Protein products were detected by SDS-PAGE and confirmed by Western blotting analysis, which showed that the yield of the Follistatin cDNA was a 63kD protein expression vector. Follistatin protein was expressed in the form of glutathione-S-transferase (GST) fusion protein in E. coli.
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Filogenia / Suínos / Proteínas Recombinantes de Fusão / Dados de Sequência Molecular / Sequência de Bases / Química / Sequência de Aminoácidos / Clonagem Molecular / Folistatina / Escherichia coli Limite: Animais Idioma: Chinês Revista: Chinese Journal of Biotechnology Ano de publicação: 2006 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Filogenia / Suínos / Proteínas Recombinantes de Fusão / Dados de Sequência Molecular / Sequência de Bases / Química / Sequência de Aminoácidos / Clonagem Molecular / Folistatina / Escherichia coli Limite: Animais Idioma: Chinês Revista: Chinese Journal of Biotechnology Ano de publicação: 2006 Tipo de documento: Artigo