Porcine follistatin cDNA cloning and expression in Escherichia coli / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 677-681, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-286228
ABSTRACT
The total RNA was extracted from porcine ovary. Porcine Follistatin cDNA was cloned by RT-PCR. Complete porcine follistatin cDNA coding sequences are presented including 1038 bp of open reading frame. The purified porcine follistatin cDNA was inserted into pGEX-4T-3 vector to construct the prokaryotic fusion protein expression vector. The recombinant expression plasmid was transformed into BL21 (DE3) and expression was induced by IPTG. Protein products were detected by SDS-PAGE and confirmed by Western blotting analysis, which showed that the yield of the Follistatin cDNA was a 63kD protein expression vector. Follistatin protein was expressed in the form of glutathione-S-transferase (GST) fusion protein in E. coli.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Filogenia
/
Suínos
/
Proteínas Recombinantes de Fusão
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Química
/
Sequência de Aminoácidos
/
Clonagem Molecular
/
Folistatina
/
Escherichia coli
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2006
Tipo de documento:
Artigo
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