Prokaryotic expression of fusion protein TAT-EDAG and study on its transduction activity / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 598-603, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-286243
ABSTRACT
HIV-TAT protein transduction domain (PTD) is a new kind of peptide that is responsible for transduction of proteins through the plasma membrane with high efficiency. Linked covalently to proteins, peptides, nucleic acid, it could transduce into nearly all kinds of cells and tissues with high efficiency and without any damages. In this study, we constructed the TAT-EDAG and TAT-GFP prokaryotic expression vectors and expressed soluble TAT-EDAG and TAT-GFP fusions in E. coli BL21 (DE3) successfully. By using the Ni-NTA-agrose purification system under the native condition we got the purified fusion proteins whose purification were higher than 90%. After desalting we found the TAT-GFP could transduced successfully into the mouse fibroblast cells and the TAT-EDAG could transduced into HL-60 cells in vitro. It will be useful to amplify the HSCs in vitro in the next step.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Transdução Genética
/
Proteínas Recombinantes de Fusão
/
Proteínas Nucleares
/
Células HL-60
/
Escherichia coli
/
Produtos do Gene tat do Vírus da Imunodeficiência Humana
/
Fibroblastos
/
Genética
/
Metabolismo
Limite:
Animais
/
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2006
Tipo de documento:
Artigo
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