The effect of oncolytic adenovirus on human umbilical vein endothelial cell / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 397-402, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-286277
ABSTRACT
The E1A gene was obtained by PCR with QBI-293A cell genome DNA as template. After enzyme digestion, the E1A gene was ligated to transfer vector pAdTrack-CMV. The positive clone pAdTrack-CMV-E1A were lineared by PmeI and co-transformed with pAdEasy-1 in BJ5183 E. coli. The recombinant adenovirus vector pAdEasy-1-pAdTrack-CMV-E1A were digested by PacI and transfected into QBI-293A cells with liposomes. The oncolytic recombinant adenovirus Ad-E1A was obtained after 7 days. The results showed that this oncolytic adenovirus Ad-E1A can replicate in ECV304 cells and inhibit growth of ECV304 cell. In addition, it also decreased the secretion of VEGF and expression of NF-kappaB of ECV304 cells, indicating that Ad-E1A have potential of inhibition of tumor metastasis.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Veias Umbilicais
/
Células Cultivadas
/
Adenoviridae
/
Regiões Promotoras Genéticas
/
Proteínas E1A de Adenovirus
/
Biologia Celular
/
Células Endoteliais
/
Proliferação de Células
/
Vírus Oncolíticos
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2006
Tipo de documento:
Artigo
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