Research of the feeding strategy in the fermentation of recombinant human fibreblast growth factor mutant / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 322-327, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-286288
ABSTRACT
Human acidic fibreblast growth factor (haFGF) was a kind of cell growth factor with wide bio-activity on cell from mesectoderm and neuro-ectoderm.In this paper, the effect of acetate concentration on the growth and expression of recombinant human acidic fibroblast growth factor mutant system E.coli BL21(DE3)/pET3C-haFGF was investigated. Four fed-batch modes batch-fed, batch-DO static balance, DO static balance-glucose starvation, and pH-static state were investigated. The accumulation of acetate during the fermentation course was effectively inhibited. The OD600nm value was about 22, after purification, the soluble rhaFGF yielded 450mg/L. During the fermentation, no special ways such as pure oxygen, pressure were adopted, thus the established process would be easily scaled up for industry purpose.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteínas Recombinantes
/
Fator 1 de Crescimento de Fibroblastos
/
Técnicas de Cultura de Células
/
Ácido Acético
/
Reatores Biológicos
/
Meios de Cultura
/
Escherichia coli
/
Proteínas Mutantes
/
Fermentação
/
Genética
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2006
Tipo de documento:
Artigo
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