The application of co-culture system on the in vitro development of bovine somatic nuclear transferred embryos / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 306-310, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-286291
ABSTRACT
To establish a co-culture system of nuclear transferred embryos in bovine, effects of co-culture cell types, passages and cryopreservation as well as addition of BFF or FBS were investigated. The results showed that embryos co-cultured with oviductal epithelial cell and granulosa cell achieved significantly higher blastocyst rate compared with the control group (P < 0.05) and co-cultured with oviductal epithelial cell had more embryo cell number than those with granulosa cell. Passages of co-culture cells significantly affected the blastocyst rate and embryo cell number (P < 0.05), and cryopreservation decreased the blastocyst rate and embryo cell number remarkably. Supplemention of BFF increased blastocyste rate significantly (P < 0.05). In conclusion, co-cultured with fresh primary oviductal epithelial cell along with addition of 10% BFF in SOFaa could improve development of nuclear transferred bovine embryo in vitro.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Técnicas de Cocultura
/
Clonagem de Organismos
/
Meios de Cultura
/
Biologia Celular
/
Desenvolvimento Embrionário
/
Embrião de Mamíferos
/
Células Epiteliais
/
Técnicas de Transferência Nuclear
/
Reprogramação Celular
/
Tubas Uterinas
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2006
Tipo de documento:
Artigo
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