Expression of TEKT4 protein decreases in the ejaculated spermatozoa of idiopathic asthenozoospermic men / 中华男科学杂志
National Journal of Andrology
;
(12): 514-517, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-286471
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the role of the TEKT4 protein in the pathogenesis of idiopathic asthenozoospermia.</p><p><b>METHODS</b>We separated and purified the ejaculated sperm from idiopathic asthenozoospermia patients and normozoospermic men by Percoll discontinuous density gradients, and detected the distribution and the expressions of TEKT4 mRNA and TEKT4 protein by RT-PCR and Western blot.</p><p><b>RESULTS</b>RT-PCR revealed that the expression of TEKT4 mRNA was significantly lower in the sperm of the idiopathic asthenozoospermia patients than in those of the normozoospermic men (0.59 +/- 0.13 vs 0.75 +/- 0.15, t = 4.325, P < 0.05), and Western blot confirmed the results of RT-PCR (0.48 +/- 0.14 vs 0.69 +/- 0.13, t = 5.939, P < 0.05).</p><p><b>CONCLUSION</b>The expression of TEKT4 is significantly decreased in the ejaculated sperm of idiopathic asthenozoospermia patients, which might be one of the causes of idiopathic asthenozoospermia.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Motilidade dos Espermatozoides
/
Espermatozoides
/
RNA Mensageiro
/
Estudos de Casos e Controles
/
Western Blotting
/
Proteínas do Citoesqueleto
/
Astenozoospermia
/
Genética
/
Metabolismo
Tipo de estudo:
Estudo observacional
Limite:
Adulto
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
National Journal of Andrology
Ano de publicação:
2012
Tipo de documento:
Artigo
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