Construction of an infectious cDNA clone derived from foot-and-mouth disease virus O/QYYS/s/06 / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 982-986, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-286613
ABSTRACT
After sequencing, we amplified and cloned foot-and-mouth disease virus (FMDV) O/QYYS/s/06 whole genome by three fragments. These three fragments were cloned into vector P43 one by one to construct recombinant plasmid P43C, which carried the full-length cDNA of FMDV O/QYYS/s/06. Then, plasmid P43C and plasmid T7 expressing T7 RNA polymerase were co-transfected into BHK-21 cells. After 48 h, we harvested the culture broth from transfected BHK-21 cells and inoculated into 2-3 day-old sucking mice. After four generation passage, the virus harvested from sucking mice was confirmed to be type O FMDV by the indirect hemagglutination test, sucking mice's neutralization test and sequencing. The results showed that we have successfully constructed the full-length cDNA clone of FMDV O/QYYS/s/06 strain.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Transcrição Gênica
/
Virologia
/
Virulência
/
DNA Viral
/
Transfecção
/
Classificação
/
Clonagem Molecular
/
DNA Complementar
/
Vírus da Febre Aftosa
/
Febre Aftosa
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2009
Tipo de documento:
Artigo
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