Biodehydrogenation of 11beta-hydroxyl melroxyprogesterone by Arthrobacter simplex UR016 in microemulsion system / 生物工程学报
Chinese Journal of Biotechnology
; (12): 892-896, 2009.
Article
em Zh
| WPRIM
| ID: wpr-286626
Biblioteca responsável:
WPRO
ABSTRACT
To improve mass transfer and enhance the yield for C(1,2) biodehydrogenation of steroid 11beta-hydroxyl medroxyprogesterone, we carried out the dehydrogenation reaction of 11beta-hydroxyl medroxyprogesterone in an oil-in-water (O/W) microemulsion by Arthrobacter simplex UR016. We studied the effects of system composition, dehydrogenation temperature and substrate concentration on microbial transformation. We formulated a suitable O/W microemulsion system with Arthrobacter simplex UR016 culture broth as aqueous phase, 10 g/L of edible oil as oil phase, 4 g/L of Tween-O80 and 7% (V/V) alcohol as surfactant and cosurfactant. The optimal dehydrogenation temperature was 33 degrees C. The results showed that in Tween-80/alcohol/edible oil/water microemulsion system, the hydrophobic steroid was solubilised and diffused effectively, with the maximum conversion rate of 88.6% at 46 h under 4 g/L substrate concentration, an increase of 66.2% compared to that in aqueous system. The C(1,2) biodehydrogenation of 11beta-hydroxyl medroxyprogesterone is more efficient in water-edible oil microemulsion system than in aqueous system.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Arthrobacter
/
Biotransformação
/
Química
/
Emulsões
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Hidrogenação
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Medroxiprogesterona
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Metabolismo
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2009
Tipo de documento:
Article