Medium optimization for enhanced production of carbonyl reductase by Candida tropicalis 104 by response surface methodology / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 863-868, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-286631
ABSTRACT
Using response surface method, we optimized the medium for the asymmetric whole cell biotransformation by Candida tropicalis 104. This strain was used for microbial reduction of 1-[3,5-bis(trifluoromethyl)phenyl] ethanone to (S)-l-[3,5-bis(trifluoromethyl)phenyl] alcohol, with enantiomeric excess(e.e.) reached more than 99.9%. Fractional factorial design was used to evaluate the effects of medium components on carbonyl reductase activity of Candida tropicalis 104. Yeast extract, glucose and NH4Cl were the most important factors among six tested variables that influence the enzyme activity for the biotransformation process. Based on the experimental results, the path of steepest ascent was undertaken to approach the optimal region of these factors. Central composite design and response surface analysis were subsequently employed for further optimization. The optimal medium for Candida tropicalis 104 was composed of (in g/L) glucose 47.14, yeast extract 13.25, NH4Cl 2.71, MgSO4.7H2O 0.4, KH2PO4 1, K2HPO4 1. Under the optimum conditions, the maximum enzyme activity of 852.75 U/L in theory and 851.13 U/L in the experiment were obtained, with an increase of 65.2% compared to the original medium components.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Meios de Cultura
/
Candida tropicalis
/
Oxirredutases do Álcool
/
Fermentação
/
Cloreto de Amônio
/
Metabolismo
/
Modelos Teóricos
Tipo de estudo:
Estudo prognóstico
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2009
Tipo de documento:
Artigo
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