Construction of anti-H5N1 virus chimeric igA antibody gene and its expression in CHO cells / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 714-719, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-286652
ABSTRACT
Abstract To express human-mouse chimeric IgA antibody directed against H5N1 virus, an anti-H5N1 chimeric IgA antibody gene was constructed by joining the light and heavy chain variable region genes and the corresponding signal peptide coding sequences of the anti-H5N1 mouse monoclonal antibody H5N1-HA with the coding sequences of the constant region of the human IgA2 heavy chain and Kappa chain respectively. Then the full-length chimeric light and heavy chain expressing plasmids pEF-IGHA9 and pEF-IGK9 were constructed and transfected into the CHO/dhfr cells. The chimeric IgA antibody expression was confirmed by ELISA, SDS-PAGE and Western blotting. The successful expression of this anti-H5N1 chimeric IgA may help to provide a stand for developing passive immunological agents for H5N1 virus infection prophylaxis.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteínas Recombinantes de Fusão
/
Imunoglobulina A
/
Cricetulus
/
Células CHO
/
Quimerismo
/
Alergia e Imunologia
/
Virus da Influenza A Subtipo H5N1
/
Genética
/
Anticorpos Monoclonais
/
Anticorpos Antivirais
Limite:
Animais
/
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2009
Tipo de documento:
Artigo
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