Fusion expression of Escherichia coli heat-labile enterotoxin B subunit gene and foot-and-mouth disease virus type O VP1 gene and immunogenicity analysis / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 560-565, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-286673
ABSTRACT
LTB gene fragment was amplified by PCR from plasmid pMDTLT, and a recombinant plasmid pETLTBVP1 was constructed by inserting LTB gene fragment into VP1 gene expression plasmid pETVP1 constructed previously. The recombinant plasmids were transformed into E. coli BL21(DE3) and induced to express by IPTG. The recombinant protein existed in the inclusion body and its molecular weight was about 39 kD proved by SDS-PAGE analysis. Western blotting showed that the fusion protein could be reacted with both anti-FMDV and anti-cholera toxin serum demonstrating the immunoactivity of the fusion protein. Strong immune responses can be induced in mice inoculated with the fusion protein intraperitoneally, and the serum antibody level is higher than that of commercial foot-and-mouth disease vaccines.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plasmídeos
/
Toxinas Bacterianas
/
Sangue
/
Proteínas Recombinantes de Fusão
/
Proteínas de Escherichia coli
/
Proteínas do Capsídeo
/
Enterotoxinas
/
Alergia e Imunologia
/
Escherichia coli
/
Fusão Gênica
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2009
Tipo de documento:
Artigo
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