Establishment of a GFP-based cellular model for screening novel proteasome inhibitors / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 452-456, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-286689
ABSTRACT
To establish a green fluorescent protein (GFP)-based cellular model for screening proteasome inhibitors from compounds including extracts from Traditional Chinese Medicinal herbs, we transfected A549 cells with lentivirus expression vector pGC-E1-ZU1-GFP, and selected clones stably expressing ZU1-GFP. The A549-ZU1-GFP cells were treated with PS-341 for 24 h, and the accumulation of GFP was analyzed by fluorescence microscope. We found that the fluorescence intensity of A549-ZU1-GFP cells treated with PS-341 was significantly increased as compared to the control. We screened for proteasome inhibitors from compounds including some from Traditional Chinese Medicinal herbs, and the data suggested a few compounds could be novel proteasome inhibitors.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Inibidores de Proteases
/
Pirazinas
/
Ácidos Borônicos
/
Medicamentos de Ervas Chinesas
/
Linhagem Celular
/
Lentivirus
/
Proteínas de Fluorescência Verde
/
Avaliação Pré-Clínica de Medicamentos
/
Bortezomib
Tipo de estudo:
Estudo diagnóstico
/
Estudo de rastreamento
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2009
Tipo de documento:
Artigo
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