Analysis of PARP10 tissue expression profile, interactive protein and UV stress reaction / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 428-434, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-286693
ABSTRACT
One pair of primers were designed and synthesized based on the cDNA sequence encoding Homo sapiens poly (ADP-ribose) polymerase family, member 10 (PARP10) reported on the GenBank. The cDNA sequence encoding PARP10 was cloned from 293FT cell by RT-PCR. Then the RT-PCR product was cloned into pCMV-Myc and pEGFP-C1 plasmids. The interaction between PARP10 and beta-actin was identified through immuno-precipitation and laser confocal microscopy. Extensive expression of PARP10 in mouse tissues was confirmed by RT-PCR. Besides, Western blotting analysis indicated that cell injury caused by UV treatment could promote the expression of PARP10. The results in this paper would benefit further study of PARP10.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Efeitos da Radiação
/
Estresse Fisiológico
/
Raios Ultravioleta
/
Distribuição Tecidual
/
Proteínas Proto-Oncogênicas
/
Actinas
/
Poli(ADP-Ribose) Polimerases
/
Domínios e Motivos de Interação entre Proteínas
/
Metabolismo
Limite:
Animais
/
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2009
Tipo de documento:
Artigo
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