Expression of imprinted genes during the course of differentiation from mouse embryonic stem cells to islet-like cells in vitro / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
;
(12): 954-958, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-286937
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of in vitro inducement on the expression of SF1-G imprinted genes, Kcnq1 and Cdkn1c during the course of differentiation from mouse embryonic stem (ES) cells to islet-like cells.</p><p><b>METHODS</b>Mouse ES cells were induced to differentiate into islet-like cells in vitro. The expression of islet specific markers was tested by RT-PCR or immunofluorescence. RT-PCR/RFLP was used to test the imprinted genes parental expression in cells at different stages.</p><p><b>RESULTS</b>Islet specific genes, such as Insulin, Glucagon, Somatostatin, IAPP and Glut2, were expressed in differentiated cells. The proteins of insulin, C-peptide and Somastatin were expressed in the final stage cells. Imprinted gene Kcnq1 and Cdkn1c were biallelicly expressed in islet-like cells.</p><p><b>CONCLUSIONS</b>Mouse ES cells can be successfully induced into islet-like cells in vitro. Gene imprinting status of Kcnq1 and Cdkn1c may be changed in differentiated cells (causing loss of imprinting) during the in vitro inducement.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Células-Tronco
/
Proteínas
/
Diferenciação Celular
/
Ilhotas Pancreáticas
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Biologia Celular
/
Células-Tronco Embrionárias Murinas
/
Insulina
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Contemporary Pediatrics
Ano de publicação:
2010
Tipo de documento:
Artigo
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