Effect of spleen tyrosine kinase on the proliferation of pulmonary vascular smooth muscle cells in rats / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
;
(12): 886-890, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-286957
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the role of spleen tyrosine kinase (Syk) in rat pulmonary vascular smooth muscle cells (PVSMCs) proliferation induced by platelet-derived growth factor-BB (PDGF-BB).</p><p><b>METHODS</b>PVSMCs from male Sprague-Dawley rats were cultured in vitro and the cells of passages 3-5 were used in the experiment. PVSMCs were stimulated by PDGF-BB and were treated with three different doses of piceatannol, a Syk selective inhibitor. Cell proliferation was assessed by methyl thiazolyl tetrazolium (MTT) assay. DNA synthesis was measured by ³H-thymidine incorporation (³H-TdR). Cellular cycle was observed by flow cytometry. Syk mRNA and protein expression were detected using real-time quantitative PCR and Western blot, respectively.</p><p><b>RESULTS</b>The expression of Syk protein of PVSMCs was significantly up-regulated following PDGF-BB stimulation. PDGF-BB stimulation dramatically increased PVSMCs proliferation. After piceatannol treatment, both Syk mRNA and protein expression decreased and the proliferation of PVSMCs was inhibited in a dose-dependent manner.</p><p><b>CONCLUSIONS</b>Syk may promote PVSMCs proliferation induced by PDGF-BB.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
Fisiologia
/
Artéria Pulmonar
/
Estilbenos
/
Proteínas Tirosina Quinases
/
Fator de Crescimento Derivado de Plaquetas
/
Células Cultivadas
/
Ratos Sprague-Dawley
/
Proteínas Proto-Oncogênicas c-sis
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Contemporary Pediatrics
Ano de publicação:
2010
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS