Study on screening differentially expressed genes in mice livers by silver staining DD-PCR / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 296-299, 2005.
Article
em Zh
| WPRIM
| ID: wpr-287029
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>AIM</b>To screen swimming-fatigue related genes in mice and lay theoretic basis for researching the molecular mechanism of fatigue.</p><p><b>METHODS</b>30 male BALB/c mice (20 +/- 2g) were divided into control group, dipping in water group and swimming-fatigue group respectively. After fatigue for swimming in swimming-fatigue group, with control group and dipping in water group, liver tissues in mice were collected. With improved silver staining mRNA differential display method, the differentially expressed genes in mice livers were screened and evaluated by reversed Northern blot. The positive segments were analyzed homology by BLAST.</p><p><b>RESULTS</b>7 of DD-ESTs were gained. Two of them only expressed in swimming-fatigue group, two down-regulated expressed, and three up-regulated. One of them was a novel gene and was accepted by GenBank, AY615302.</p><p><b>CONCLUSION</b>Seven DD-ESTs in swimming-fatigue mice were gained by silver staining mRNA differential display method.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Natação
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RNA Mensageiro
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Dados de Sequência Molecular
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Reação em Cadeia da Polimerase
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Coloração pela Prata
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Perfilação da Expressão Gênica
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Fadiga
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Genética
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Fígado
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Metabolismo
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2005
Tipo de documento:
Article