Distribution and efficiency of pEGFP-N1 plasmid after its transfection in cardiomyocytes / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 112-114, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-287084
ABSTRACT
<p><b>AIM</b>To study the distribution and efficiency of pEGFP-N1 plasmid after its transfection in cardiomyocytes.</p><p><b>METHODS</b>The neonatal rat cardiomyocytes were cultured. According to the different grow period of neonatal rat cardiomyocytes, the distribution and efficiency of pEGFP-N1 plasmid after its transfection in cardiomyocytes were studied.</p><p><b>RESULTS</b>The efficiency was significantly increased after pEGFP-N1 plasmid transfection in one-day cardiomyocytes. The distribution of EGFP was in the cytoplasm and nucleus.</p><p><b>CONCLUSION</b>The efficiency of pEGFP-N1 plasmid after its transfection in cardiomyocytes was related to the grow period of neonatal rat cardiomyocytes. The distribution of EGFP was in the cytoplasm and nucleus.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plasmídeos
/
Transfecção
/
Células Cultivadas
/
Ratos Wistar
/
Miócitos Cardíacos
/
Proteínas de Fluorescência Verde
/
Genética
/
Animais Recém-Nascidos
/
Metabolismo
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2005
Tipo de documento:
Artigo
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