Whole-cell recording of potassium and sodium currents in cortical, hippocampal, and sympathetic ganglia neurons / 中国应用生理学杂志

ABSTRACT

<p><b>AIM</b>To establish the culturing of cortical, hippocampal and sympathetic ganglia neurons derived from neonatal rats and the whole-cell recording techniques of potassium and sodium currents in cultured neurons.</p><p><b>METHODS</b>Cortex, hippocampus and sympathetic ganglion were isolated from neonatal rats (1-3 days) and digested by trypsin (0.125%). The neurons were cultured in polylysine covered petri dish (O35 mm) with DEME medium(high glucose) for seven days for electrophysiological experiments.</p><p><b>RESULTS</b>The neurons cultured by this method were in good conditions and could be sealed easily in whole-cell recording. I(Na), I(A), and I(K) could be recorded respectively in cultured neurons by different recording paradigms in Ca2+ -free extracellular solution.</p><p><b>CONCLUSION</b>It is a practicable and economical method for electrophysiological study of nervous system.</p>