Construction and identification for cell strain of anti-human seminal plasma phospholipase A2 monoclonal antibody / 中华男科学杂志
National Journal of Andrology
; (12): 173-177, 2002.
Article
em Zh
| WPRIM
| ID: wpr-287255
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVES</b>To establish and evaluate the anti-human seminal plasma phospholipase A2 (PLA2) monoclonal antibody (McAb).</p><p><b>METHODS</b>After having been separated and purified from human seminal plasma by PEG precipitation, Sephacryl S-300 column chromatography, DEAE-Sephadex A-25 column chromatography and HA column chromatography, PLA2 was regarded as an antigen to immune BALB/C mouse to produce anti-human seminal plasma PLA2 McAb. The PLA2 McAb sensitivity and specificity were performed by ELISA technique and Western-blot analysis, respectively.</p><p><b>RESULTS</b>The molecular weight of PLA2 depurated with 245 fold purification from human seminal plasma was about 34,900, while the sensitivity and typing of its McAb were 1:5(6)-1:5(8) and IgM (kappa) with a satisfied Western-Blot results.</p><p><b>CONCLUSIONS</b>The PLA2, which had not been reported in international and domestic papers, may be a new type of PLA2. The establish of its McAb will provide significant tools for the research of the relationship between PLA2 in human seminal plasma and male fertility.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Fosfolipases A
/
Sêmen
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Química
/
DEAE-Dextrano
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Eletroforese em Gel de Poliacrilamida
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Alergia e Imunologia
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Fosfolipases A2
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Metabolismo
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Peso Molecular
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Anticorpos Monoclonais
Limite:
Humans
Idioma:
Zh
Revista:
National Journal of Andrology
Ano de publicação:
2002
Tipo de documento:
Article