Role of mitochondria in Tubeimoside I-mediated apoptosis in human cervical carcinoma HeLa cell line / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1935-1939, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-287291
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the role of mitochondria in TBMS1-medited apoptosis of human cervical carcinoma HeLa cell line.</p><p><b>METHOD</b>Mitochondrial transmembrane potentia (deltapsim) was assayed by flow cytometry. Apoptotic induction by TBMS1 was determined by gel electrophoresis of fragmented DNA. Cytochrome c (Cyt c) was detected by Western blotting.</p><p><b>RESULT</b>The results showed that TBMS1 induced apoptosis in HeLa cells, that TBMS1 decreased deltapsim and facilitated Cyt c release, and that TBMS1 induced apoptosis of HeLa cells dose-and time-dependently in accordance with increase of cytosolic Cyt c. TBMS1 had direct effect on isolated rat mitochondria which induced a time- and dose-dependent release of Cyt c from mitochondria. The results also showed that CsA protected partly HeLa cells from the effect of TBMS1.</p><p><b>CONCLUSION</b>The mitochondrial apoptosis pathway has effects on TBMS1 induced human cervical carcinoma HeLa cell line apoptosis.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Fisiologia
/
Plantas Medicinais
/
Saponinas
/
Triterpenos
/
Mitocôndrias Hepáticas
/
Células HeLa
/
Química
/
Ciclosporina
/
Ratos Sprague-Dawley
Limite:
Animais
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2005
Tipo de documento:
Artigo
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