Effect of osteopontin silencing by lentivirus-mediated delivery of siRNA on glioma cell invasion and apoptosis / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 525-528, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-287383
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of osteopontin silencing on the invasion and apoptosis of U251 cells.</p><p><b>METHODS</b>The invasion, apoptosis and levels of uPA, MMP-2 and MMP-9 were determined by invasion assay, flow cytometry, Western blot and real-time fluorescence quantitative PCR respectively.</p><p><b>RESULTS</b>Osteopontin small interference RNA (siRNA) inhibited osteopontin expression and cell invasion, promoted apoptosis in U251 cells. In addition, the expression of Bcl-2, uPA, MMP-2 and MMP-9 was decreased, while Bax level was elevated.</p><p><b>CONCLUSION</b>Osteopontin siRNA can inhibit U251 cells invasion via the down-regulation of uPA, MMP-2 and MMP-9 levels, and promote apoptosis through induction of Bax expression and inhibition of Bcl 2 level. It suggests that osteopontin plays an important role in human glioma progression.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Regulação Neoplásica da Expressão Gênica
/
Apoptose
/
Técnicas de Transferência de Genes
/
Lentivirus
/
Inativação Gênica
/
RNA Interferente Pequeno
/
Linhagem Celular Tumoral
/
Osteopontina
/
Vetores Genéticos
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Medical Genetics
Ano de publicação:
2009
Tipo de documento:
Artigo
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