Effect of synchronous perfusion of NaN3 in changes in content of cholinergic neurotransmitter in medial prefrontal cortex and hippocampal extra-cellular fluid / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 488-492, 2014.
Artigo
em Chinês
| WPRIM
| ID: wpr-287560
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of synchronous perfusion of specific respiratory chain complex IV inhibitor sodium azide (NaN3) in brain on rat ventromedial prefrontal cortex (mPFC) and acetylcholine (ACh) and choline (Ch) contents in hippocampal extra-cellular fluid, and establish the AD rat model induced by mitochondrial acute injury.</p><p><b>METHOD</b>The synchronous dual-probe dual-channel brain microdialysis sampling technology was applied to synchronously perfuse modified Ringer's solution containing NaN3 (50 micro mol L-1) and neostigmine (2 micro mol L-1) into mPFC and hippocampus of conscious, freely moving normal rats, and continuously collect dialysates from different encephalic areas. Dynamic contents of ACh and Ch were determined by high performance liquid chromatography-post-column immobilized enzyme reactor-electrochemical process.</p><p><b>RESULT</b>ACh and Ch contents in mPFC extracellular fluid of normal rats were higher than that in hippocampus. During the process of perfusion, NaN3 could significantly reduce ACh in mPFC/hippocampal extra-cellular fluid, but remarkably increase Ch, and constantly inhibit the recovery of ACh and Ch contents in mPFC/hippocampus.</p><p><b>CONCLUSION</b>The synchronous perfusion of NaN3in rat mPFC and hippocampus can injure functions of the cholinergic nerve projection area, and cause the acute AD model with ACh and Ch metabolic disorders. This model can be used in pathogenetic and pharmacological studies.</p>
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Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Perfusão
/
Farmacologia
/
Fatores de Tempo
/
Acetilcolina
/
Colina
/
Ratos Sprague-Dawley
/
Córtex Pré-Frontal
/
Neurotransmissores
/
Azida Sódica
/
Biologia Celular
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2014
Tipo de documento:
Artigo
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